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cfx384 real time pcr instrument  (Bio-Rad)


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    Bio-Rad cfx384 real time pcr instrument
    Cfx384 Real Time Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 10101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx384+touch+real+time+pcr+detection+system/CFX384+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc13156779-104-28-32
    Average 99 stars, based on 10101 article reviews
    cfx384 real time pcr instrument - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention.
    Article Snippet: Total RNA (1 μg) was converted into first-strand cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Monza, Italy). .. Quantitative RT-PCR was performed, with 16 ng cDNA, in CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Milan, Italy) using a SYBR Green PCR Master Mix (Bio-Rad Laboratories, Milan, Italy) and the synthesized https://doi.org/10.3390/biology15090719 primers (Thermo Fisher Scientific, Waltham, MA, USA) listed in Table 1. ..

    Article Title: Hepatoprotective effects of epigallocatechin gallate and glutathione on aflatoxin B1-induced subacute liver injury in ducklings
    Article Snippet: A reverse transcription kit (TaKaRa, Dalian, China) was used to synthesize cDNA. .. After that, TB Green® Premix Ex Taq TM II (TaKaRa, Dalian, China) was used to perform RT-qPCR with CFX384 Touch Real-Time PCR Detection System (Bio-Rad, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention.
    Article Snippet: Total RNA (1 μg) was converted into first-strand cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Monza, Italy). .. Quantitative RT-PCR was performed, with 16 ng cDNA, in CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Milan, Italy) using a SYBR Green PCR Master Mix (Bio-Rad Laboratories, Milan, Italy) and the synthesized https://doi.org/10.3390/biology15090719 primers (Thermo Fisher Scientific, Waltham, MA, USA) listed in Table 1. ..

    Article Title: Hepatoprotective effects of epigallocatechin gallate and glutathione on aflatoxin B1-induced subacute liver injury in ducklings
    Article Snippet: A reverse transcription kit (TaKaRa, Dalian, China) was used to synthesize cDNA. .. After that, TB Green® Premix Ex Taq TM II (TaKaRa, Dalian, China) was used to perform RT-qPCR with CFX384 Touch Real-Time PCR Detection System (Bio-Rad, USA). ..

    Article Title: Diagnostic accuracy of plasma cell-free DNA qPCR for Schistosoma haematobium assessed by Bayesian latent class analysis in a cohort of pregnant women from Lambaréné, Gabon.
    Article Snippet: Each qPCR reaction mix (20 μL total volume) contained 4 μmol/L of primers, 14 μL of DNA, 4 μmol/L of probe and 4 μL of AptaTaq Genotyping Master (Roche Diagnostics Deutschland GmbH, Mannheim, Germany). .. The adjusted protocol included a hot start at 95 °C for 10 min, followed by 45 cycles of 95 °C for 30 s and annealing at 60 °C for 45 s. All qPCR assays were performed on the CFX384 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, California, United States). ..

    Article Title: Turnip mosaic virus co-opts host RNA methylation to orchestrate plant infection
    Article Snippet: RT-qPCR was performed using SsoAdvancedTM Universal SYBR ® Green Supermix (1725271, BioRad), following the supplier specifications for designing the thermocycle. .. Samples were run on a CFX384 Touch Real-Time PCR Detection System (BioRad). ..

    Article Title: Immune infiltration is associated with distinct transcriptional states and metabolic profiles in chordomas
    Article Snippet: .. The qPCR reactions were performed using iQ SYBR Green Supermix (Bio-Rad) and run on a C1000 Touch Thermal Cycler equipped with the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Lung-targeted cytokine-coding RNA-lipoplexes induce T and NK cell-mediated anti-tumor immune response
    Article Snippet: Primers used in quantitative Real-Time PCR (qRT-PCR) were designed to bind specifically mRNA-related transcripts of the target genes of interest, assisted by “Oligo Analysis Tool” ( https://eurofinsgenomics.eu/en/ecom/tools/oligo-analysis/ ), “OligoAnalyzer” ( https://eu.idtdna.com/calc/analyzer ), “Primer-BLAST” ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ), “UCSC In-Silico-PCR” ( https://genome.ucsc.edu/cgi-bin/hgPcr ) and “UNAFold - mFold DNA Folding Form” ( http://www.unafold.org/mfold/applications/dna-folding-form.php ). .. The assays were validated using a BioRad CFX384 Touch Real-Time PCR Detection System and BioRad’s SsoAdvanced Universal SYBR Green Supermix determining the optimal annealing temperature by comparative runs, including melting curve analysis. .. Correct PCR product size was controlled by capillary gel electrophoresis (QIAGEN QIAxcel or Agilent Fragment Analyzer).


    SYBR Green Assay:

    Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention.
    Article Snippet: Total RNA (1 μg) was converted into first-strand cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Monza, Italy). .. Quantitative RT-PCR was performed, with 16 ng cDNA, in CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Milan, Italy) using a SYBR Green PCR Master Mix (Bio-Rad Laboratories, Milan, Italy) and the synthesized https://doi.org/10.3390/biology15090719 primers (Thermo Fisher Scientific, Waltham, MA, USA) listed in Table 1. ..

    Article Title: Immune infiltration is associated with distinct transcriptional states and metabolic profiles in chordomas
    Article Snippet: .. The qPCR reactions were performed using iQ SYBR Green Supermix (Bio-Rad) and run on a C1000 Touch Thermal Cycler equipped with the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Lung-targeted cytokine-coding RNA-lipoplexes induce T and NK cell-mediated anti-tumor immune response
    Article Snippet: Primers used in quantitative Real-Time PCR (qRT-PCR) were designed to bind specifically mRNA-related transcripts of the target genes of interest, assisted by “Oligo Analysis Tool” ( https://eurofinsgenomics.eu/en/ecom/tools/oligo-analysis/ ), “OligoAnalyzer” ( https://eu.idtdna.com/calc/analyzer ), “Primer-BLAST” ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ), “UCSC In-Silico-PCR” ( https://genome.ucsc.edu/cgi-bin/hgPcr ) and “UNAFold - mFold DNA Folding Form” ( http://www.unafold.org/mfold/applications/dna-folding-form.php ). .. The assays were validated using a BioRad CFX384 Touch Real-Time PCR Detection System and BioRad’s SsoAdvanced Universal SYBR Green Supermix determining the optimal annealing temperature by comparative runs, including melting curve analysis. .. Correct PCR product size was controlled by capillary gel electrophoresis (QIAGEN QIAxcel or Agilent Fragment Analyzer).

    Polymerase Chain Reaction:

    Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention.
    Article Snippet: Total RNA (1 μg) was converted into first-strand cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Monza, Italy). .. Quantitative RT-PCR was performed, with 16 ng cDNA, in CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Milan, Italy) using a SYBR Green PCR Master Mix (Bio-Rad Laboratories, Milan, Italy) and the synthesized https://doi.org/10.3390/biology15090719 primers (Thermo Fisher Scientific, Waltham, MA, USA) listed in Table 1. ..

    Synthesized:

    Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention.
    Article Snippet: Total RNA (1 μg) was converted into first-strand cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Monza, Italy). .. Quantitative RT-PCR was performed, with 16 ng cDNA, in CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Milan, Italy) using a SYBR Green PCR Master Mix (Bio-Rad Laboratories, Milan, Italy) and the synthesized https://doi.org/10.3390/biology15090719 primers (Thermo Fisher Scientific, Waltham, MA, USA) listed in Table 1. ..

    Amplification:

    Article Title: Transcriptomic profiling and RANKL/RANK/OPG-mediated osteoclastogenesis in zebrafish larvae under simulated microgravity conditions
    Article Snippet: Each reaction contained 300 nM primers, 5 μL SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories) and 4 μL of cDNA. .. The amplifications were performed on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) according to the following thermal conditions: initial denaturalization 95 °C for 2 min followed by 40 cycles of amplification, consisting of denaturation at 95 °C for 10 s, annealing at 60 °C for 20 s and extension at 72 °C for 20 s. Secondary denaturation was then performed at 95 °C for 10 s followed by a melt curve analysis from 65 °C to 95 °C in 0.5 °C increments for 5 s each. ..



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